Plasmid

Part:BBa_K1391144:Design

Designed by: James Anderson   Group: iGEM14_MIT   (2014-10-30)


pEXPR TRE:BACE2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal XbaI site found at 4525
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4900
    Illegal PstI site found at 5523
    Illegal PstI site found at 5966
    Illegal PstI site found at 6774
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal NheI site found at 3210
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4900
    Illegal PstI site found at 5523
    Illegal PstI site found at 5966
    Illegal PstI site found at 6774
    Illegal NotI site found at 7079
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal BglII site found at 6317
    Illegal BamHI site found at 2854
    Illegal BamHI site found at 3328
    Illegal BamHI site found at 7274
    Illegal XhoI site found at 3538
    Illegal XhoI site found at 7059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal XbaI site found at 4525
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4900
    Illegal PstI site found at 5523
    Illegal PstI site found at 5966
    Illegal PstI site found at 6774
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5641
    Illegal EcoRI site found at 5688
    Illegal EcoRI site found at 6237
    Illegal XbaI site found at 4525
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4900
    Illegal PstI site found at 5523
    Illegal PstI site found at 5966
    Illegal PstI site found at 6774
    Illegal NgoMIV site found at 2297
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 4024
    Illegal NgoMIV site found at 5353
    Illegal NgoMIV site found at 5809
    Illegal AgeI site found at 3456
    Illegal AgeI site found at 4638
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1121
    Illegal BsaI.rc site found at 5284
    Illegal SapI site found at 38
    Illegal SapI site found at 3214
    Illegal SapI site found at 7506


Design Notes

No particular considerations were taken into account.


Source

BACE2 was purchased commercially and then assembled with the promoter part pENTR TRE by Gateway.

References